This has been solved, see bellow:
Code:
title Kali
set ISO=/iso/kali-linux-2016.2-i386.iso
find --set-root %ISO%
uuid () && call set UUID=%^?%
map %ISO% (0xff) && map --hook
root (0xff)
kernel /live/vmlinuz boot=live noconfig=sudo username=root hostname=kali live-media-path=live fromiso=/dev/disk/by-uuid/%UUID%%ISO% live-config.locales=fr_FR.utf-8 live-config.keyboard-layouts=us,fr live-config.keyboard-options=grp:ctrl_shift_toggle persistence rw #toram=filesystem.squashfs
initrd /live/initrd.img
"toram" argument transfers the ISO to RAM and mounts the source disk as read/write.
Persistence mode, as explained here, can be enabled by creating an ext3/ext4 primary type partition with label 'persistence', then create in that partition a file named 'persistence.conf' with the following content: / union